I haven't seen a tier list for a game like this so I figured I'll try making one right now. Feel free to discuss and add your opinion about certain character rankings.

Top:
Zoe
Mousy McNuts
Professor von Brown

High:
ISO
Mr. Truffles
Tyrone
Lord Peet
Gullarp

Middle:
Pector El Pollo
Marky Kat
Billy Ray Logg

Low:
Fox Claw
Ivar

Bottom:
Unit 51

Marky Kat should be higher

My list:

Top:
Zoe
Truffles
Peet

High:
Marky
ISO
Tyrone

Middle:
Mousy
Von Brown
Fox Claw
Pector

Low:
Logg
Unit 51

Trash:
Ivar/Gullarp (That floaty jump just makes them huge targets.)

 Durty Biscuit wrote:

Middle:
Mousy

Sorry, can't take anything you said seriously because of that.

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First in world to 100% Fat Princess | Gears 1 fan
They call me Hodoken because I'm downright fierce!

Ok but having over 52000 kills and 5000ish wins I think I know who goes were.  And the only reason Mousy is higher on your list is because of his secondary fire.  Problem is if you can't move fast enough to keep up with me your secondary fire won't do anything to me, and thanks to Mousy's weak armor you will die in 2-3 well placed shotgun blasts.

I played this game at the highest for a while, I think I can give a pretty accurate list.

Top:
Mr. Truffles
Marky Kat
Professor Von Brown
Tyrone

High:
Mousy McNuts
Zoe

Middle:
ISO
Pector El Pollo
Lord Peet
Gullarp

Low
Fox Claw
Billy Ray Log
Ivar

Bottom:
Unit 51

Wow you apparently don't understand most characters are perfectly balanced.
But still, here is my list..

Top:
Billy Ray Logg
Fox Claw
Unit 51
Marky Cat

High:
ISO
Mr. Truffles
Tyrone
Zoe

Middle:
Pector El Pollo
Lord Peet
Professor von Brown

Low:
Mousy McNuts

Bottom:
Ivar & Gullarp (Worst Characters Ever)

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Xbox 360: $349.99
Watching Noobs Complain: Priceless

God:
Truffles

Upper:
Prof. Von Brown
Lord Peet
Marky Cat

Middle:
Tyrone
ISO
Zoe
Rat Nub
Pector El Pollo

Lower:
Tree
Fox Claw

Trash: (thx 4 teh pr0pr titl)
Unit ****Face
DLC Characters